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The LOD of the RT-NASBA assay. The RT-NASBA assay could detect 95%, 30%, and 20% of samples with 200, 100, and 50 copies/mL of SARS-CoV-2 RdRp gene, respectively. The assay could detect 100%, 30%, and 15% of samples with 200, 100, and 50 copies/mL of SARS-CoV-2 N gene, respectively. Thus, the LOD of the assay is 200 copies/mL
