SADS-CoV infection in primary swine IECs. (A) PLAC8 mRNA expression of SADS-CoV-RFP–infected IECs in DM or EM at 72 and 96 hpi. (B) Viral growth of SADS-CoV-RFP on primary swine IEC in DM or EM. (C) Representative images of SADS-CoV-RFP–infected IECs in DM and EM at 72 hpi. (D) PLAC8 and glycocalyx integrity in neonatal and adult pig ileum. Neonatal and adult swine ileal sections of naïve pigs were stained with PLAC8 (red), UEA lectin (green), and DAPI (blue). Scale bar is 50 µm. Error bars represent ± 1 SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by Student's t test.