TABLE 1.
Bacterial strains used in this study
| Straina | Characteristics and origin | Source |
|---|---|---|
| Sinorhizobium meliloti | ||
| Rm41 | Wild type from Medicago sativa; Europe | Bánfalvi et al. |
| Rm1021 | Str-resistant derivative of wild-type strain RCR2011 | Walker et al. |
| NRG23 | Wild type from M. sativa; North America | Olsen et al. |
| NRG34 | Wild type from M. sativa; North America | Philom Bios |
| NRG43 | Wild type from M. sativa; North America | Olsen et al. |
| NRG53 | Wild type from M. sativa; North America | Olsen et al. |
| NRG133 | Wild type from M. sativa; North America | Olsen et al. |
| NRG185 | Wild type from M. sativa; North America | Olsen et al. |
| NRG247 (Balsac)b | Wild type from M. sativa; North America | Olsen et al. |
| NRG282 | Wild type from M. sativa; North America | Olsen et al. |
| NRG286 | Wild type from M. sativa; North America | Olsen et al. |
| Sinorhizobium sp. strain NGR234 | Wild type from Lablab purpureus; New Guinea | Trinick |
| Sinorhizobium fredii | ||
| HH103 | Wild type from soil; Hubei province, China | Dowdle and Bohlool |
| HH303 | Wild type from soil; Hubei province, China | Dowdle and Bohlool |
| USDA191 | Wild type from soil; Shanghai province, China | Keyser et al. |
| USDA192 | Wild type from Glycine soja; China | Keyser et al. |
| USDA196 | Wild type from soil; Hubei province, China | Dowdle and Bohlool |
| USDA197 | Wild type from soil; Hubei province, China | Dowdle and Bohlool |
| USDA201 | Wild type from Glycine max cv. Peking; China | Keyser et al. |
| USDA205 | Wild type from G. max cv. Peking; China | Keyser et al. |
| USDA208 | Wild type from G. max cv. Peking; China | Keyser et al. |
| USDA257 | Wild type from G. max cv. Peking; China | Keyser et al. |
a
S. meliloti NRG34, S. meliloti NRG185, and S. meliloti NRG247 (Balsac) were included in this study because the MAbs were raised against whole cells of these strains (10) (see Table 2). S. meliloti NRG23, NRG43, NRG53, NRG133, NRG286, and NRG289 were chosen because they were negative for MAb 10 in the previous study (11), which was the exception. S. meliloti Rm1021, S. meliloti Rm41, and S. fredii USDA257 were used because they are the most commonly studied microsymbionts in serogroup A. S. fredii USDA205 and Sinorhizobium sp. strain NGR234 were analyzed because they are in serogroup B.
b
S. meliloti NRG247 is referred to as Balsac in the report of Olsen et al. (11).