Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) | Gr64f-Gal4 (II) | Kwon et al., 2011 | BDSC:57669; FLYB:FBti0162679 |
|
| Genetic reagent (D. melanogaster) | Gr64f-Gal4 (III) | Kwon et al., 2011 | BDSC:57668; FLYB: FBti0162678 |
|
| Genetic reagent (D. melanogaster) | Gr64f-LexA (III) | Miyamoto et al., 2012 | ||
| Genetic reagent (D. melanogaster) | Gr66a-Gal4 (II) | Scott et al., 2001 | ||
| Genetic reagent (D. melanogaster) | Gr66a-LexA (III) | Thistle et al., 2012 | ||
| Genetic reagent (D. melanogaster) | Ppk28-Gal4 (II) | Cameron et al., 2010 | ||
| Genetic reagent (D. melanogaster) | Ppk28-LexA (III) | Thistle et al., 2012 | ||
| Genetic reagent (D. melanogaster) | Ir94e-Gal4 (attp2) | Croset et al., 2016 | BDSC:81246; FLYB:FBti0202323 |
|
| Genetic reagent (D. melanogaster) | csChrimsonReporter/Optogenetic effector,20xUAS- csChrimson::mVenus in attP18 | Klapoetke et al., 2014 | BDSC:55134; FLYB:FBst0055134 | |
| Genetic reagent (D. melanogaster) | UAS-Syt-HA;; | Robinson et al., 2002 | ||
| Genetic reagent (D. melanogaster) | UAS-P2X2 (chr III) | Lima and Miesenböck, 2005 | BDSC:91222; FLYB:FBst0091222 |
|
| Genetic reagent (D. melanogaster) | UAS-ArcLight (attp2) | Cao et al., 2013 | BDSC:51056; FLYB:FBst0051056 |
|
| Genetic reagent (D. melanogaster) | LexAop-GCaMP6s (attp5) | Chen et al., 2013 | BDSC:44589; FLYB:FBst0044589 |
|
| Genetic reagent (D. melanogaster) | LexAop-GCaMP6s (attp1) | Chen et al., 2013 | BDSC:44588; FLYB:FBst0044588 |
|
| Genetic reagent (D. melanogaster) | LexAop-Gal80 (X) | Thistle et al., 2012 | ||
| Genetic reagent (D. melanogaster) | UAS-CD8::tdTomato (chr X) | Thistle et al., 2012 | ||
| Genetic reagent (D. melanogaster) | UAS-CD8::tdTomato (II) | Thistle et al., 2012 | ||
| Antibody | Anti-Brp (mouse monoclonal) | DSHB, University of Iowa, USA | DSHB:Cat# nc82; RRID:AB_2314866 |
1/500 |
| Antibody | Anti-GFP (rabbit polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# A11122; RRID:AB_221569 |
1/1000 |
| Antibody | Anti-GFP (chicken polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# A10262; RRID:AB_2534023 |
1/1000 |
| Antibody | Anti-dsRed (rabbit polyclonal) | Takara Bio | Takara Bio:Cat# 632496; RRID: AB_10013483 |
1/1000 |
| Antibody | Anti-rabbit Alexa Fluor 488 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# A11034; RRID:AB_2576217 |
1/100 |
| Antibody | Anti-chicken Alexa Fluor 488 (goat polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# A11039; RRID:AB_2534096 |
1/100 |
| Antibody | Anti-rabbit Alexa Fluor 568 (goat polyclonal) |
Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# A11036; RRID:AB_10563566 |
1/100 |
| Antibody | Anti-mouse Alexa Fluor 647 (goat polyclonal) |
Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# A21236; RRID:AB_2535805 |
1/100 |
| Chemical compound, drug | Denatonium benzoate | MilliporeSigma | MilliporeSigma:Cat# D5765; CAS:3734-33-6 |
|
| Chemical compound, drug | Caffeine | MilliporeSigma | MilliporeSigma:Cat# C53; CAS:58-08-2 |
|
| Chemical compound, drug | Sucrose | Thermo Fisher Scientific | Thermo Fisher Scientific:Cat# AAA1558336; CAS:57-50-1 |
|
| Chemical compound, drug | Polyethylene glycol (MW 3350) | MilliporeSigma | MilliporeSigma:Cat# P4338; CAS:25322-68-3 |
|
| Chemical compound, drug | All-trans-retinal | MilliporeSigma | MilliporeSigma:Cat# R2500; CAS:116-31-4 |
|
| Software, algorithm | Fiji | Schindelin et al., 2012 | RRID:SCR_002285 | http://fiji.sc/ |
| Software, algorithm | CATMAID | Schneider-Mizell et al., 2016 | RRID:SCR_006278 | https://catmaid.readthedocs.io/ |
| Software, algorithm | R Project for Statistical Computing | R Development Core Team, 2018 | RRID:SCR_001905 | https://www.r-project.org/ |
| Software, algorithm | NeuroAnatomy Toolbox | Jefferis and Manton, 2017 | RRID:SCR_017248 | https://github.com/jefferis/nat |
| Software, algorithm | Python | Python Software Foundation | RRID:SCR_008394 | https://www.python.org/ |
| Software, algorithm | Jupyter Notebook | Project Jupyter | RRID:SCR_018315 | https://jupyter.org/ |
| Software, algorithm | Slidebook | Intelligent Imaging Innovations | RRID:SCR_014300 | https://www.intelligent-imaging.com/slidebook |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 | https://www.graphpad.com/ |
| Software, algorithm | Cytoscape | Shannon et al., 2003 | RRID:SCR_003032 | https://cytoscape.org/ |
| Software, algorithm | Computational Morphometry Toolkit | Rohlfing and Maurer, 2003 | RRID:SCR_002234 | https://www.nitrc.org/projects/cmtk/ |