Skip to main content
. 2022 Jun 6;11:e71947. doi: 10.7554/eLife.71947

Figure 2. Validation of essential genes.

(A) Growth curve of Mycobacterium abscessus (Mab) strains with sgRNAs targeting canonical essential genes. (B) Growth curve of Mab strains with sgRNAs targeting uniquely essential Mab genes.

Figure 2.

Figure 2—figure supplement 1. Validation of uniquely essential Mycobacterium abscessus (Mab) genes.

Figure 2—figure supplement 1.

(A) Transformation of CRISPRi plasmids carrying sgRNAs targeting canonical and (B) uniquely essential genes in Mab. Equal volume of transformations was plated on +/-ATc (anhydrotetracycline) plates.