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. 2022 Jun 6;11:e71947. doi: 10.7554/eLife.71947

Figure 5. Knockdown of penicillin-binding protein and hypothetical lipoprotein (PBP-lipo) disrupts formation of FtsZ rings.

(A) Microscopy images of N-terminally tagged mRFP-PBP-lipo and C-terminally tagged FtsZ-mNeonGreen. ‘Merged’ images show overlay of red and green channels. (B) (Left) Demograph of mRFP-PBP-lipo. (Right) Quantification of septal fluorescence signal across increasing cell lengths. (C) (Left) Demograph of mRFP-PBP-lipo and FtsZ-mNeonGreen. (Right) Fluorescence signal arranged by increasing cell length. (D) Images of GFP-FtsZ expressed from its natural promoter in the setting of PBP-lipo knockdown.

Figure 5.

Figure 5—figure supplement 1. mRFP-PBP-lipo is a functional fusion protein.

Figure 5—figure supplement 1.

(A) Schematic of mRFP-PBP-lipo-strep fusion protein. (B) Western blot using ɑ-strep antibody on strains expressing PBP-lipo-strep and mRFP-PBP-lipo-strep. (C) Growth curve showing complementation with mRFP-PBP-lipo fusion proteins.
Figure 5—figure supplement 1—source data 1. Western blot detecting presence of mRFP-PBP-lipo-strep.