Figure 5. The Dcp1–Dcp2 decapping enzyme forms distinct decapping complexes in vivo with the decapping activators Edc3, Dhh1, Upf1, and Pat1.

Two-hybrid assays, as in Figure 1, were used to dissect the molecular basis of five Dcp2-bridged interactions between Dcp1 and Dhh1, Upf1, or Pat1, and between Edc3 and Upf1 or Pat1. (A) Dcp1:Dhh1, Dcp1:Upf1, Dcp1:Pat1, Edc3:Upf1, and Edc3:Pat1 interactions in tester strains harboring different gene deletions or truncations of decapping factors. (B) Dcp1:Dhh1, Dcp1:Upf1, and Dcp1:Pat1 interactions in tester strains harboring Dcp2 C-terminal truncations in EDC3 and edc3Δ cells. (C) Edc3:Upf1 and Edc3:Pat1 interactions in tester strains harboring Dcp2 C-terminal truncations in EDC3 and edc3Δ cells. (D) Three different decapping complexes inferred from two-hybrid analyses in A and B. (E) Two different decapping complexes inferred from two-hybrid analyses in A and C.