Chemical compound, drug |
[α-32P]-dCTP |
Perkin Elmer |
Blu513Z |
|
Chemical compound, drug |
5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL) |
USB |
7240-90-6 |
40 µg/ml |
Chemical compound, drug |
Phenol |
Fisher |
A92-500 |
|
Chemical compound, drug |
Phenol:choroform:IAA (25:24:1) |
Ambion |
AM9732 |
|
Chemical compound, drug |
Herring Sperm DNA |
Promega |
D1815 |
|
Chemical compound, drug |
Geneticin (G418 Sulfate) |
Gibco |
11811-023 |
400 µg/ml |
Chemical compound, drug |
Hygromycin B |
Roche |
10843555001 |
200 µg/ml |
Peptide, recombinant protein |
Taq DNA polymerase |
Roche |
04-728-874-001 |
|
Commercial assay or kit |
QuikChange XL Site-Directed Mutagenesis Kit |
Agilent Technologies |
200,519 |
|
Commercial assay or kit |
Random Primed DNA labeling Kit |
Roche |
11-004-760-001 |
|
Strain, strain background (Escherichia coli) |
DH5α |
Invitrogen |
11319-019 |
Electrocompetent cells |
Strain, strain background (Saccharomyces cerevisiae) |
Supplementary file 1
|
This paper |
Yeast strains used in this study |
See Materials and methods |
Genetic reagent (plasmid) |
Supplementary file 2
|
This paper |
Plasmids used in this study |
See Materials and methods |
Genetic reagent (plasmid) |
Supplementary file 4
|
This paper |
DNA probes used in this study |
See Materials and methods |
Sequence-based reagent |
Supplementary file 3
|
This paper |
Oligonucleotides used in this study |
See Materials and methods |
Software, algorithm |
Multi-Gauge software |
Fujifilm |
Science lab 2005 |
|
Software, algorithm |
GraphPad Prism 9 for Windows |
GraphPad Software, LLC |
https://www.graphpad.com
|
|
Other |
Zeta-Probe Blotting Membranes |
BioRad |
1620159 |
He et al., 2008
|
Other |
Mini Quick Spin RNA Columns |
Roche |
11-814-427-001 |
He et al., 2008
|