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. 2022 May 23;11:e74410. doi: 10.7554/eLife.74410

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Chemical compound, drug [α-32P]-dCTP Perkin Elmer Blu513Z
Chemical compound, drug 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-GAL) USB 7240-90-6 40 µg/ml
Chemical compound, drug Phenol Fisher A92-500
Chemical compound, drug Phenol:choroform:IAA (25:24:1) Ambion AM9732
Chemical compound, drug Herring Sperm DNA Promega D1815
Chemical compound, drug Geneticin (G418 Sulfate) Gibco 11811-023 400 µg/ml
Chemical compound, drug Hygromycin B Roche 10843555001 200 µg/ml
Peptide, recombinant protein Taq DNA polymerase Roche 04-728-874-001
Commercial assay or kit QuikChange XL Site-Directed Mutagenesis Kit Agilent Technologies 200,519
Commercial assay or kit Random Primed DNA labeling Kit Roche 11-004-760-001
Strain, strain background (Escherichia coli) DH5α Invitrogen 11319-019 Electrocompetent cells
Strain, strain background (Saccharomyces cerevisiae) Supplementary file 1 This paper Yeast strains used in this study See Materials and methods
Genetic reagent (plasmid) Supplementary file 2 This paper Plasmids used in this study See Materials and methods
Genetic reagent (plasmid) Supplementary file 4 This paper DNA probes used in this study See Materials and methods
Sequence-based reagent Supplementary file 3 This paper Oligonucleotides used in this study See Materials and methods
Software, algorithm Multi-Gauge software Fujifilm Science lab 2005
Software, algorithm GraphPad Prism 9 for Windows GraphPad Software, LLC https://www.graphpad.com
Other Zeta-Probe Blotting Membranes BioRad 1620159 He et al., 2008
Other Mini Quick Spin RNA Columns Roche 11-814-427-001 He et al., 2008