Figure 1.

CAF-induced resistance to 5-FU in GC cells. (A) GC cells were co-cultured with fibroblast culture-conditioned media and treated with 5-FU and the IC₅₀ value was determined. (B) Western blot analysis showing the expression levels of the indicated proteins following 5-FU treatment with and without co-culture with CAFs in various GC cell lines. (C) Representative photomicrograph demonstrating the morphological changes in GC cells following 5-FU treatment for 72 h co-culture with or without CAFs. 5-FU, 5-fluorouracil; CAF, cancer-associated fibroblast; GC, gastric cancer. Scale bar, 200 µm.