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. 2022 May 26;61(1):85. doi: 10.3892/ijo.2022.5375

Figure 2.

Figure 2

Identification of the JAK2/STAT3 axis as a specific communicator between CAFs and GC cells. (A) Schematic diagram of secretome analysis of conditioned media and transcriptome and western blot analysis for MKN1 cells, MKN74 cells and SNU668 cells. IL-6, IL-8 and CCL2 were secreted at higher levels in co-cultured with CAFs relative to MKN74 cells and SNU668 cells cultured alone. These factors were all associated with the JAK/STAT3 signal transduction pathway. Gene set enrichment analysis revealed that the IL-6/JAK/STAT3 signaling-related genes were enriched in MKN74 cells and SNU668 cells co-cultured with CAFs (MKN-74 cell enrichment score, 1.83, nominal P<0.001, FDR Q=0.001, FWER P=0.006; SNU668 cell enrichment score, 1.456; nominal P<0.01, FDR Q=0.01, FWER P=0.145). (B) KEGG pathway analysis expressed increased genes between no co-culture and co-culture with CAFs in MKN1 cells, MKN74 cells and SNU668 cells. (C) Western blot analysis showing the expression levels of the indicated proteins after co-culture with CAFs in MKN1 cells, MKN74 cells and SNU668 cells. (D) Western blot analysis showing the expression levels of the indicated proteins following 5-FU treatment with or without co-culture with CAFs in MKN1 cells, MKN74 cells and SNU668 cells. 5-FU, 5-fluorouracil; CAF, cancer-associated fibroblast; GC, gastric cancer; CCL2, C-C motif chemokine ligand 2; KEGG, Kyoto Encyclopedia of Gene and Genomes; IL-6, interleukin-6; IL-8, interleukin-8; JAK, Janus-activated kinase; STAT3, signal transducer and activator of transcription 3.