Figure S1.

Mutations in the VPS13C locus in VPS13C^KO clones 1 and 2. (A) Live HeLa cells expressing full-length VPS13C^Halo with VAPA-GFP and mCherry-Rab7a^T22N (not depicted). Enlarged images of boxed areas shown to the right. (B) Percentage abundance of each mutated allele of 48 bacterial colonies sequenced. The HeLa cell genome is known to be aneuploid. (C) Live WT and VPS13C^KO (clone 1) HeLa cells expressing TFEB-GFP. (D) Quantification of nuclear to cytosolic GFP intensity from C, n = 175 cells across three biological replicates. (E) IB showing lack of VPS13C band in VPS13C^KO cells and return of band in repaired VPS13C^Rescue clones. Images from the same blot as Fig. 1 G. Scale bars, 20 μm. Inset scale bars, 5 μm. **, P < 0.01. Data were compared using a two-sided t test. Error bars represent ±SD. Source data associated with this figure can be found at https://doi.org/10.5281/zenodo.6416363. Source data are available for this figure: SourceData FS1.