TABLE 1.
Primers used in the study
| Primer ID | Sequence (5′‐3′)* | Usage |
|---|---|---|
| Cas9_F | GCCTATTCTGTGCTGGTGGT | Presence/absence of transgene |
| Cas9_R | TCCAGGTAGTGCTTGTGCTG | |
| GhU6F1 | TGTGCCACTCCAAAGACATCA | Analyzing the integrated gRNA |
| gRNAR1 | ATGCACGCGCTAAAAACGGA | |
| GhU6F2 | TGGTCAGGACGTGGTAGCATA | Sequencing PCR product generated by GhU6F1/gRNAR1 |
| miR482ac_F | GTTCTCTCTCACTCTCACCCC | Editing events of gRNA31/37/38 |
| miR482ac_R | CCGATTTAACTTACCGAGTGC | |
| miR482b_F | GAAGAAGTATGGGTGTTAGCCG | Editing events of gRNA34/35/36 |
| miR482b_R | CATTTTCACCAAGGTTCCCTGC | |
| miR482d1_F | TACTTTCTCTTTCTCCTCACC | Editing events of gRNA39 |
| miR482d1_R | GGTTTACCGATTTCACTTAC | |
| miR482d2_F | TTTCTACTCATTTTCTGTGGC | Editing events of gRNA39 |
| miR482d2_R | ATATCTCCGCCGCTCATAACC | |
| miR482d3_F | TTAGACTGTTGTGAAGGAGGA | Editing events of gRNA39 |
| miR482d3_R | CAAATATAGTGCTAACGTCCG | |
| miR482d4_F | GACGCATAATTCTCTGTGGC | Editing events of gRNA39 |
| miR482d4_R | GGTAAGGTTTACCGATTTCAC | |
| miR482e_F | AAGGTAAGGAAAGCCGCCAC | Editing events of gRNA40/41 |
| miR482e_R | CTTTCCAACTCCTCCATTCC | |
| miR482f_F | AGGCGATTGGTATGAGCACC | Editing events of gRNA44 |
| miR482f_R | GCGAAGGTGCAGGCATGAAA | |
| miR482g_F | ATATGCAATGAAGTTGATTTTGCC | Editing events of gRNA46 |
| miR482g_R | GAGTAAAAGGAAAAGCAAACC | |
| miR482h_F | CCTTCTCTTCGTTTGCCCAT | Editing events of gRNA48 |
| miR482h_R | GTTCTCTGAAAATCACAACCAG | |
| miR482k_F | CTTATGAAAGTTGGATGACC | Editing events of gRNA49/50 |
| miR482k_R | CATACCTTCTTCCATATACC | |
| miR482l_F | TTGAAACATAAAGGACCAGC | Editing events of gRNA51 |
| miR482l_R | CCTTACTTTCTTCCGTTTCC |