TABLE 2.
gRNAs used in the study
| gRNA ID | gRNA + PAM | Target gene | Off‐target (mismatch) |
|---|---|---|---|
| gRNA31 | GGAGTTTATGGAAGTGGGATGGG | miR482a_A04 | miR482c_D04 (1 MM) |
| gRNA32 | AGAACTGGAAATAAGTGGTATGG | miR482a_A04 | miR482c_D04 (1 MM) |
| gRNA33 | TGGGTGAGGGGGTCAGACAATGG | miR482b_D13 | miR482b_scaf3700_A13 (1 MM) |
| gRNA34 | GCCTACTCCACCCATGCCACTGG | miR482b_A13/D13 | |
| gRNA35 | TGGGTGAGGGGGTAAGACAATGG | miR482b_scaf3700_A13 | miR482b_D13 (1 MM) |
| gRNA36 | TTAGGAGGAGGAGGAACACCAGG | miR482b_scaf3700_A13 | miR482b_D13 (1 MM) |
| gRNA37 | ATCCCACTTCCAAAAACTCCCGG | miR482c_D04 | miR482a_A04 (1 MM) |
| gRNA38 | ATAAGTGGAATGGGAGGAGTAGG | miR482c_D04 | miR482a_A04 (1 MM) |
| gRNA39 | ATCAGTGGAATGGGAGGAATTGG | miR482d (all 19 loci) | |
| gRNA40 | GTCTTCGGCTGTGGTTGATTCGG | miR482e_A05/D05 | |
| gRNA41 | ATCGTAGGCATGGGTGGAATCGG | miR482e_D05 | miR482e_A05 (1 MM) |
| gRNA42 | ATAGTAGGCATGGGTGGAATCGG | miR482e_A05 | miR482e_D05 (1 MM) |
| gRNA43 | GGAAACGGAGAGAGAGTTTCTGG | miR482f_A03 | miR482f_D02 (1 MM) |
| gRNA44 | TGGGGGGCATAGGAAAGATCTGG | miR482f_A03/D02 | |
| gRNA45 | AGTAATAGGTATGGGGGGTATGG | miR482g_A03/D02 | |
| gRNA46 | ATGAAGGGTATGGGAGGTGTTGG | miR482g_A03/D02 | |
| gRNA47 | GTTGGAGGTGTGGGAGAGTTGGG | miR482h_A05/D05 | |
| gRNA48 | ATCAAGGGTATGGGAGGGGTTGG | miR482h_A05/D05 | |
| gRNA49 | GAAAGGGAGATTGAAGTTTCTGG | miR482k_A07 | miR482k_D07 (1 MM) |
| gRNA50 | CTGGAAATCATTGGAATTGGAGG | miR482k_A07/D07 | |
| gRNA51 | ATTACAGGAATGGGGTCTTTGGG | miR482l_A12/A12 | |
| gRNA52 | ATAAATTGAAGTTCTTCCCGAGG | miR482l_A12/A12 |