TABLE 3.

Analysis of steady-state chemostat cultures of strain TCE1, Desulfitobacterium sp. strain PCE1, and D. hafniense grown under PCE- or 3Cl-4OH-PA-limiting conditions in the presence of excess lactate or formate as the electron donor

Organism	μmax (h−1)	Steady-state dilution rate (h−1)	Substrates consumed (mM)				Products formed (mM)								Protein concn (mg liter−1)	Growth yieldb	Dechlorination velocityc	Specific rate of dechlorinationd
			Lactate	PCE	Formate	3-Cl-4OH-PA	Acetate	Butyrate	TCE	cis-DCE	1,1-DCE	trans-DCE	4OH-PAa	Chloride
Strain TCE1	0.24	0.015	14.1	10.9			15.2		0.002	10.8	0.04			23.0	69	1.5	0.34	0.08
		0.05	16.1	12.5			15.8		NDe	ND	ND			21.0	71	1.7	1.1	0.25
		0.062	17.9	8.8			24.0		ND	ND	ND			20.3	104	2.6	1.2	0.2
		0.071	15.7	9.7			13.1		0.04	9.6	0.04			17.6	64	1.8	1.2	0.33
		0.081	12.8	7.7			17.9		ND	ND	ND			15.3	56	1.9	1.2	0.37
		0.223	9.6	4.8			9.1		ND	ND	ND			14.3	39	1.4	3.2	1.4
Desulfitobacterium sp. strain PCE1	0.14	0.02	26.6	27.7			26.0	0.6	25.3	1.3		0.8		26	60	1.7	0.52	0.14
		0.04	11.2	10.6			15.5	0	8.9	0.9		0.6		11	48	2.2	0.44	0.15
		0.06	20.3	13.4			11.2	0.1	11.1	1.4		0.8		12	55	2.3	0.72	0.22
		0.08	14.3	17.5			11.1	0	15.7	1.1		0.7		14	62	2.2	1.1	0.31
	0.14	0.02	26.0			10.1	32.4	0.8					9.5	11	81	3.7	0.23	0.05
		0.05	15.0			10.1	10.1	0.2					9.2	10	66	3.3	0.5	0.13
		0.11	19.0			10.3	8.3	0.2					9.6	11	60	2.8	1.2	0.34
	0.21	0.03			28.2	18.7	0.4	0					17.5	22	78	1.8	0.66	0.14
		0.06			25.2	18.7	0	0.2					17.1	21	71	1.7	1.3	0.3
		0.09			26.3	18.3	0	0.1					17.3	17	80	2.4	1.5	0.32
D. hafniense	0.052	0.009	35.0			8.0	36.0						ND	7.8	64	4.1	0.07	0.02
		0.03	16.6			8.0	16.2						7.6	8.0	48	3.0	0.22	0.09

^a4OH-PA, 4-hydroxyphenylacetate. 

^bExpressed in grams of protein formed per mole of electrons transferred. 

^cExpressed in millimoles of Cl⁻ released per liter per hour. 

^dExpressed in micromoles of Cl⁻ released per minute per milligram of protein. 

^eND, not determined.