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. 2022 Jun 6;13(6):528. doi: 10.1038/s41419-022-04984-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — For each experiment, immunoprecipitation was carried out with protein extracts from the indicated HEK293T cell lines using anti-Flag antibody and then the precipitate was analyzed by western blot analysis using antibodies indicated in the panels. a Extracts from cells co-transfected with Flag-EphA2, V5-EGFR and HA-K-Ras (WT, G12V, and Q61L) plasmids. b Extracts from cells transfected with Flag-tagged EphA2, V5-tagged EGFR along with or without Myc-tagged myr-AKT (constitutively active). c Extracts from cells co-transfected with Flag-tagged Ephexin1 with or without HA-tagged K-Ras^G12V plasmids. d Extracts from cells transfected with Flag-tagged EphA2 WT or S897D plasmids. e Extracts from cells co-transfected with Flag-tagged Ephexin1 and V5-tagged EphA2 that were treated with EGF (100 ng/ml) after 16 h serum starvation. Western blot analysis was carried out using anti-EphA2 pSer897 antibody. f Extracts from cells co-transfected with Flag-tagged EphA2 WT or S897D, V5-tagged EGFR with or without shEphexin1 plasmids.