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. 2022 Jun 6;12:9113. doi: 10.1038/s41598-022-11743-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum likelihood phylogeny inferred from IQ-TREE analysis of concatenated ddRAD dataset for (a) the Largemouth Bass complex and (b) the Smallmouth Bass complex. Population structure (K = 20) inferred from snmf analysis for each species complex are shown to the right of the phylogeny. In the clade delimiting the Largemouth Bass complex (a), population structure assignments and branches leading to specimens from the vicinity of Charleston, South Carolina (type locality of Micropterus salmoides) are highlighted in orange. Green vertical bars indicate populations of genetic admixture between M. nigricans and M. salmoides that are identified in TreeMix analysis. Red arrows indicate the phylogenetic placement and population structure assignment of a specimen sampled from Cuatro Ciénegas. See Supplementary Fig. S1 for a completely annotated phylogeny tip labels and bootstrap supports values. Illustrations © Joseph R. Tomelleri, used with permission.