TABLE 1.
Bacteria, fungi, plasmids, and cosmids used in this study
| Organism, plasmid, or cosmid | Relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| E. coli DH5α | F− φ80d/lacZ M15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK−, mK+) supE44 λ−thi-l gyrA96 relA1 | BRLa |
| Bacillus calidolactis | 5 | 5 |
| A. nidulans strains | ||
| R21 | yA2 pabaA1 | 9 |
| G191 | pabaA1 pyrG89 uaY fwA1 | 1 |
| AXB4A | biA1 bga0 argB2::pAXB4A ArgB+, WG355 derivative carrying pAXB4A | 5 |
| AXB4A2 | biA1 pyrG89 bga0 argB2::pAXB4A ArgB+, WG355 derivative carrying pAXB4A | 31 |
| AXTII9 | biA1 bga0 argB2::(pAXB4A)2 ArgB+, WG355 derivative carrying pAXB4A | 5 |
| Prg-1 | prgA1 biA1 bga0 argB2::(pAXB4A)2 ArgB+, AXTII9 derivative | 6 |
| Prg-6 | prgB1 biA1 bga0 argB2::(pAXB4A)2 ArgB+, AXTII9 derivative | 6 |
| K103 | prgA1 biA1 pyrG89 fwA1 argB2::(pAXB4A)2 ArgB+ | This study |
| PrgA1CA | K103 transformed with a cosmid derived from the pCAP2 library, PyrG+ PrgA1+ | This study |
| PrgA1CB | K103 transformed with a cosmid derived from the pCAP2 library, PyrG+ PrgA1+ | This study |
| PrgA1CC | K103 transformed with a cosmid derived from the pCAP2 library, PyrG+ PrgA1+ | This study |
| DE58 | ΔsuAprgA1 biA1 pyrG89 bga0 argB2::pAXB4A ArgB+ Pyr-4+ AXB4A2 carrying a deletion of the suAprgA1 gene | This study |
| PrgA1T | K103 transformed with a cosmid derived from the pCAP2 library, PyrG+ | This study |
| Plasmids and cosmids | ||
| pAXB4A | acvA-uidA ipnA-lacZ argBb | 5 |
| cosCA.1 | Cosmid rescued from PrgA1CA | This study |
| cosCA.2 | Cosmid rescued from PrgA1CA | This study |
| cosCA.3 | Cosmid rescued from PrgA1CA | This study |
| pKTB1 | pyr-4+ | K. Then Bergh |
| pKO1 | This study | |
| pKO2 | This study | |
| pKO3 | This study |
a
BRL, Bethesda Research Laboratories, Gaithersburg, Md.
b
The mutant argB allele can complement chromosomal argB mutations.