FIGURE 1.
Blocking P2X7 receptor with AZ 10606120 prevents vascular dysfunction in rat aorta induced by IL‐1β. Freshly isolated rat aortae were subjected to buffer (Ctrl) or IL‐1β (50 ng/ml) in the presence or absence of AZ 10606120 (AZ; 10 µM) for 2 h. (a) Rat aortae were suspended in a muscle bath, contracted with PE, and treated with escalating doses of carbachol (CCH; 10−8–10−5 M). The percent relaxation was determined as a change to the maximal PE‐induced contraction. (b) Percent relaxation to 5 × 10−7 M CCH shows AZ 10606120 prevents dysfunction of endothelial‐dependent relaxation induced by IL‐1β (n = 6). Statistical analysis was performed using one‐way ANOVA with Tukey's multiple comparisons. Graphs represent mean ± SEM