FIGURE 5.

Blocking P2X7 receptor with AZ 10606120 does not prevent increased circulating inflammatory markers during murine sepsis. Mice were subjected to cecal slurry (CS) to induce sepsis in the presence or absence of 1‐h pre‐treatment with AZ 10606120 (AZ; 10 µg/g, SQ) for 24 h. Circulating inflammatory markers (plasma), including (a) IL‐1β, (b) IL‐6, (c) TNF‐α, (d) IFN‐γ, (e) CXCL1, or (f) IL‐10, were not reduced with AZ 10606120 treatment in mice subjected to CS. Moreover, several levels of inflammatory markers (IL‐1β, IL‐6, TNF‐α, CXCL1) were worsened with AZ 10606120 treatment. Statistical analysis was performed using one‐way ANOVA with Tukey's multiple comparisons. Graphs represent mean ± SEM (n = 7–13)