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(A) calcoaceticus induces inflammatory responses in intestinal organoids. (A) Inside-out organoids incubated with live fluorescently tagged A. calcoaceticus and immunostained with the apical marker phospho-Ezrin. qPCR analysis of organoids after 3 h of incubation examining expression of (B) Tnf, (C) IL-18, (D) Mcp-1, (E) IL-1alpha, and (F) Muc1, (G) Muc13, (H) Muc2, and (I) Relmβ.
