Fig. 7.

Antigen specific T cell response elicited by E2-E1-LNP mRNA vaccine is CD8⁺ T cell dominant. Splenocytes from each group (n ​= ​5–6) were harvested and used for quantifying antigen specific CD8⁺IFN-γ⁺ T cells or CD4⁺IFN-γ⁺ T cells by flow cytometry. A Representative results of mRNA vaccine induced CD8⁺IFN-γ⁺ T cells. B Representative results of mRNA vaccine induced CD4⁺IFN-γ⁺ T cells. C–E Percentage of CD8⁺IFN-γ⁺ T cells stimulated with sE2-E1 protein (C), E2EP3 peptide (D) and E1_328-345 peptide (E). F–H Percentage of CD4⁺IFN-γ⁺ T cells stimulated with sE2-E1 protein (F), E2EP3 peptide (G) and E1_328-345 peptide (H). Statistical differences were determined using t-test (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001).