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Purification and specific activity of chlorite dismutase from (per)chlorate-reducing strain CKB
| Prepn | Vol (ml) | Protein concn (mg/ml) | Total protein (mg) | Sp act (U/mg)a | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 30.0 | 27.72 | 831.6 | 35.0 | 1 |
| Q-Sepharose | 120.0 | 0.777 | 93.24 | 398.0 | 11.4 |
| Hydroxyapatite | 144.0 | 0.200 | 28.80 | 640.0 | 18.3 |
| Phenyl-Sepharose | 48.0 | 0.080 | 3.84 | 1,102.0 | 32.0 |
| Superdex 200 | 10.0 | 0.307 | 3.07 | 1,928.0 | 55.0 |
One unit was defined as 1 μmol of sodium chlorite dismutated per min.
