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. 2022 May 24;10:899869. doi: 10.3389/fcell.2022.899869

FIGURE 2.

FIGURE 2

Apoptosis and necroptosis levels in miniature pigs with acute kidney injury induced by ischemia-reperfusion. (A) Representative images of TUNEL staining of kidney tissue from each group. Scale bar = 100 µm. (B) Quantification and plot of the TUNEL stained images. (C) Photomicrographs illustrating immunofluorescence staining of phospho-MLKL. Scale bar = 200 µm. (D) Quantification and plot of the immunofluorescence integrated density of phospho-MLKL. (E) Porcine kidney tissue lysates from four groups were subject to immunoblot analysis with specific antibodies against cleaved-caspase 3, MLKL, phospho-MLKL, RIPK3 and phospho-RIPK3. Expression levels of cleaved-caspase 3 (F), MLKL (H) and RIPK3 (J) were quantified by densitometry analysis and then normalized with GAPDH. The phosphorylation levels of MLKL (G) and RIPK3 (I) were calculated quantitatively by densitometry. Data are means ± sem.*p < 0.05; **p < 0.01 versus sham controls.