FIGURE 5.

Exosomes down-regulated apoptosis and necroptosis levels in pigs with acute kidney injury induced by ischemia-reperfusion. (A) Representative images of TUNEL staining of kidney tissue from four groups. Scale bar = 100 µm. (B) Quantification and plot of the TUNEL stained images. (C) Photomicrographs illustrating immunofluorescence staining of phospho-MLKL. Scale bar = 200 µm. (D) Quantification and plot of the Immunofluorescence integrated density of phospho-MLKL. (E) Porcine kidney tissue lysates from four groups were subject to immunoblot analysis with specific antibodies against cleaved-caspase 3, MLKL, phospho-MLKL. Expression levels of cleaved-caspase 3 (F) and MLKL (H) were quantified by densitometry analysis and then normalized with GAPDH. The phosphorylation level of MLKL (G) was calculated quantitatively by densitometry. Data are means ± sem.*p < 0.05; **p < 0.01 versus sham controls.