Table 4.

Effect of porcine bile acids on egg quality in laying hens¹.

Items		Bile acids (mg/kg)					SEM	P -Value
	Time (d)	0	60	300	600	3,000		ANOVA	Linear	Quadratic
Eggshell strength (N)	28	33.72	35.17	33.61	33.72	32.72	0.71	0.882	0.550	0.836
	56	31.69	31.60	34.98	34.80	32.15	0.96	0.645	0.841	0.384
Eggshell percentage (%)	28	8.62	8.77	9.18	9.02	9.13	0.08	0.107	0.164	0.098
	56	8.91	9.08	8.96	8.66	8.81	0.07	0.439	0.269	0.398
Eggshell thickness (mm)	28	0.34	0.35	0.34	0.35	0.35	0.01	0.825	0.454	0.705
	56	0.37	0.39	0.40	0.40	0.39	0.01	0.111	0.627	0.158
Albumen height (mm)	28	5.94	6.39	6.29	6.11	6.63	0.14	0.594	0.250	0.497
	56	6.13	6.32	5.29	6.12	6.03	0.13	0.141	0.803	0.837
Haugh units	28	74.41	79.72	78.88	77.11	79.25	1.19	0.630	0.591	0.862
	56	77.24	78.69	68.88	76.58	77.46	1.27	0.119	0.928	0.762
Yolk color	28	4.82a	4.67a	4.71a	4.44ab	4.00b	0.07	<0.001	<0.001	<0.001
	56	4.44a	4.39a	4.19ab	4.19ab	3.83b	0.06	0.011	0.002	0.013
Yolk percentage (%)	28	26.83	26.86	26.76	26.48	25.91	0.18	0.403	0.028	0.088
	56	27.27	27.57	28.00	26.91	26.26	0.20	0.069	0.003	0.020

¹Data represent mean values of 6 replicates of 5 eggs each. SEM means standard error of the mean.

^{a, b}Means within a row column with no common superscripts differ significantly (P < 0.05).