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. 2022 Feb 19;9:uhac045. doi: 10.1093/hr/uhac045

Figure 1.

Figure 1

Interaction verification between lpu-miR171a and lpu-miR171b and the LpSCL6-II and LpSCL6-I target genes. a GUS histochemical staining. pRI101-GUS refers to the empty vectors. miR171a is a vector containing mature miR171a. miR171b is a vector containing mature miR171b. SCL-I-GUS is a vector containing LpSCL6-I. SCL-II-GUS is a vector containing LpSCL6-II. Scale bar = 1 cm. b GUS enzyme activity. c Verification results of lpu-miR171a and lpu-miR171b and the LpSCL6-II and LpSCL6-I target genes. 35S mini pro::LUC refers to the empty vectors. 35S::miR171a is a vector containing mature miR171a. 35S::SCL6 II is a vector containing LpSCL6-II. 35S::miR171a + 35S::SCL6 II refers to the observation result of the mixed injection of a vector containing mature miR171a and a vector containing LpSCL6-II. 35S::miR171b is a vector containing mature miR171b. 35S::SCL6 I is a vector containing LpSCL6-I. 35S::miR171b + 35S::SCL6 I refers to the observation result of the mixed injection of a vector containing mature miR171b and a vector containing LpSCL6-I. 35S mini pro::LUC + 35S::SCL6 I refers to the observation result of the mixed injection of empty vectors and a vector containing LpSCL6-I. 35S mini pro::LUC + 35S::SCL6 II refers to the observation result of the mixed injection of empty vectors and a vector containing LpSCL6-II.