Figure 1.

Identification of LINC01234 as a metabolism-related lncRNA

(A) The expression level of ASS1 in cancerous tissues and non-cancerous tissues derived from patients with HCC by UALCAN (http://ualcan.path.uab.edu/).

(B) Overall survival analysis based on ASS1 levels by Kaplan-Meier Plotter (http://kmplot.com/analysis/) (n = 364, log rank test, two-sided).

(C) Venn diagrams of overlapped lncRNA between ASS1-associated lncRNAs and top30 differentially expressed lncRNAs in TCGA LIHC.

(D) The correlation analysis between LINC01234 expression level and ASS1 expression level according to TCGA dataset.

(E) The LINC01234 expression and ASS1 expression were evaluated by RT-qPCR in a cohort of patients with HCC (PKUCancer cohort).

(F) Expression level of LINC01234 in HCC tissues and paired adjacent non-tumoral tissues based on the TCGA dataset.

(G) The expression level of LINC01234 and ASS1 in HCC tissues and paired adjacent non-tumoral tissues was detected by RT-qPCR.

(H) Overall survival and disease-free survival analyses based on LINC01234 level were analyzed using data from PKUCancer cohort.

(I) Overall survival and disease-free survival analyses based on LINC01234 levels were analyzed using data from TCGA-LIHC cohort.

(J) RT-qPCR detection of LINC01234 expression in the cytoplasmic and nuclear fractions.

(K) The subcellular localization of LINC01234 was determined by FISH.

(L) The expression of LINC01234 was evaluated by RT-qPCR and the expression of ASS1 was detected by western blot in multiple HCC cells.