Figure 4.

LINC01234 inhibits the expression of ASS1

(A) KEGG pathway enrichment analysis for the DEGs between LINC01234-upregulated and -downregulated HCC.

(B) Huh7 cells were stably transfected with LINC01234 shRNAs. RT-qPCR was performed for analyzing the mRNA levels of the indicated genes.

(C) HepG2 or Huh7 cells were stably transfected with LINC01234 shRNAs. Then, total RNAs were extracted from the cells and RT-qPCR was performed as indicated.

(D) Cells stably expressing LINC01234 were harvested. Then, total RNAs were extracted from the cells and RT-qPCR was performed as indicated.

(E) Cellular aspartate level was determined in the indicated cells.

(F) Total proteins were extracted from LINC01234-depleted cells and western blot was performed for the indicated proteins.

(G) LINC01234-overexpressed cells were harvested and lysed. The total proteins were extracted and subjected to western blot using the indicated antibodies. See also Figure S1.