Figure 5.

LINC01234 suppresses the transcriptional activation of ASS1

(A) Luciferase activity in HEK293T cells transfected with indicated vectors was evaluated. Data are presented as the relative ratio luciferase activity to renilla activity.

(B) An RIP analysis was performed to detect p53-bound LINC01234 using anti-p53 antibody.

(C) HCC cells were transfected with the indicated vectors. Then, the whole cell lysates were subjected to co-immunoprecipitation using anti-p53 antibody.

(D) The colocalization of LINC01234 and p53 was determined. The nuclei were stained with DAPI. Scale bar, 10 μm.

(E) The subcellular colocalization of LINC01234 and p53 was determined by RIP assay.

(F) HepG2 and Huh7 cells were transfected as indicated. Western blot was performed to determine the protein level of p53.

(G) Chromatin immunoprecipitation (ChIP)-qPCR analyses were employed to analyze the occupancy on the promoter of ASS1, P21, and Puma in HepG2 cells.

(H) An RT-qPCR analysis was used to detect the p21 and puma levels in HepG2 cells.

(I) Cells transfected with the indicated vectors were harvested. The cell lysates were subjected to western blot using the indicated antibodies. Data are shown as mean ± standard deviation. See also Figure S2.