Figure 3.

Different doses of antagomir for miR-200c-3p can target specific cardiac cell types

(A) Study design. Wild-type (WT) mice were injected with Cy3-labeled antagomir-200c-3p on 3 consecutive days with 5 mg/kg BW (red arrows) or once with 80 mg/kg BW (blue arrow). After 1 week, hearts were harvested for cardiac tissue and cell isolation. (B) Percentage of Cy3 labeling in isolated cardiac cell analysis. (C and D) Percentage of cells positive for Cy3 in different cardiac cell populations after administration of a (C) low or (D) high dose of antagomir. (E) Representative images of heart sections from untreated or antagomir-treated mice: staining of cell nuclei with Hoechst (first column), detection of Cy3-labeled antagomir (second column), detection of CMs with α-actinin antibody (third column), Isolectin B4 for endothelial cell detection (fourth column), and a merge of all different staining (fifth column). ∗p < 0.05 versus CMs (error bars indicate SEM).