Figure 1. Tumour necrosis factor (TNF) quantification after bacterial exposure in U-drug treated or control PBMCs.

Peripheral blood mononuclear cells (PBMCs) (400,000) were either treated with U-drug for 24 hrs or left untreated. A-D: Post U-drug treatment PBMCs were stimulated with LPS, Salmonella typhimurium and AIEC and supernatants were collected at 0-, 2-, 4- and 6-hour time points for TNF quantification by ELISA. Statistical significance was determined using two-way ANOVA, multiple comparisons; Bonferroni’s multiple comparisons test (*p<0.01 and **** p<0.0001).