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. 2022 Apr 27;119(18):e2201859119. doi: 10.1073/pnas.2201859119

Fig. 4.

Fig. 4.

Alterations in Hippo pathway signaling in Brap LKO mice and characterization of Brap/Mst2 interaction. (A) Yap phosphorylation in livers of WT and Brap LKO mice. (B) Yap phosphorylation in HepG2 cells overexpressing Brap WT or an E3 ligase–deficient mutant BRAP (BrapCA). (C) Western blot of Hippo pathway proteins in WT and Brap LKO mice. (D) Relative expression via qPCR of Yap target genes in livers of WT and Brap LKO mice. (E) mRNA expression (from RNA-sequencing counts) of Hippo pathway genes. (F) Immunoprecipitation (IP) with anti-Mst2 antibody or IgG control of HEK293T cell lysates. (G) Immunoprecipitation with anti-Myc antibody of lysates from HEK239T cells transfected with Myc-Mst2 and either GFP or Flag-Brap. Alb, albumin. *P < 0.05; **P < 0.05; ***P < 0.0005.