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. 2022 Apr 4;23(6):e54105. doi: 10.15252/embr.202154105

Figure 2. PLTs constrain the WOX5 expression domain.

Figure 2

  • A–F
    Representative FM4‐64‐stained Arabidopsis roots (grey) expressing pWOX5::mVenus‐NLS (green) in Col, plt2, plt3, and plt2, plt3 double mutant background in longitudinal (A‐D), or transversal (E‐F) optical sections. (E′, F′) Analysis of representative images in (E) and (F) in Imaris to detect and count individual expressing nuclei. (E″, F″) Overlay of 10 roots (biological replicates) showing the area of detected fluorescence (high levels in red, low levels in blue) in Col and plt2, plt3 double mutant roots.
  • G
    Number of nuclei (biological replicates) expressing pWOX5::mVenus‐NLS in Col and plt2, plt3 double mutant roots summarized in box and scatter plots.
  • H
    Area of WOX5 expression in µm2 in Col and plt2, plt3 double mutant roots summarized in box and scatter plots.

Data information: (G, H) Box = 25‐75% of percentile, whisker = 1.5 interquartile range, − = median, □ = mean value, X = minimum/maximum. (G, H) Kruskal–Wallis ANOVA with subsequent Dunn’s test (G) or one‐way ANOVA and post‐hoc Holm–Sidak multiple comparisons test was used to test for statistical significance (H). Asterisks indicate statistically significant differences (α = 0.01). Number of analyzed roots (n) (biological replicates) is indicated for each genotype and results from three technical replicates per genotype. Scale bars represent 10 µm; NLS = nuclear localization signal.