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. 2022 Apr 4;23(6):e54105. doi: 10.15252/embr.202154105

Figure EV3. Subnuclear localization and PrD prediction of WOX5, PLT1, PLT2, PLT3, and PLT4.

Figure EV3

  • A, D, G, J, M
    Representative images of (sub‐)nuclear localization of WOX5‐mV (A), PLT1‐mV(D), PLT2‐mV (G), PLT3‐mV (J) and PLT4‐mV (m) in transiently expressing N. benthamina epidermal cells. Scale bars represent 5 µm.
  • B, E, H, K, N
    Schematic representation of WOX5 (B), PLT1 (E) PLT2 (H), PLT3 (K), and PLT4 (N) protein domains. The areas in red are predicted prion‐like domains (PrDs), analyzed using the PLAAC prediction tool. Yellow areas are polyQ stretches (defined as more than three Qs in a row) in the PLT3 and PLT4 amino acid sequence.
  • C, F, I, L, O
    Protein sequences of WOX5 (C) PLT1 (F), PLT2 (I), PLT3 (L), and PLT4 (O). The red highlighted sequences are the predicted prion‐like domains (PrDs). mV = mVenus fluorescent protein; PrD = prion‐like domain; EAR = Ethylene‐responsive binding factor‐associated repression domain; WUS = WUSCHEL box; AP2 = APETALA2 domain; NLS = nuclear localization signal.

Source data are available online for this figure.