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. 2022 Apr 4;23(6):e54105. doi: 10.15252/embr.202154105

Figure EV4. PLT3‐WOX5 interaction in the Arabidopsis root.

Figure EV4

  • A–A′
    Representative image of the SCN in a lateral root of an Arabidopsis reporter line expressing WOX5‐mV (A) and PLT3‐mCh (A′) in Col‐0 background driven by their respective endogenous promoters. The TFs localize to overlapping domains (A″). Blue arrowheads mark QC cells, green arrowheads mark CSCs. Scalebars represent 10 µm.
  • B
    Fluorescence Lifetime Imaging (FLIM) results of experiments performed in the SCN of lateral Col‐0 roots expressing either only WOX5‐mV (donor‐only) or both WOX5‐mV and PLT3‐mCh driven by their respective endogenous promoters. Number of analyzed roots (n) (biological replicates) is indicated for each genotype and results from 4 technical replicates. Donor fluorescence lifetimes in ns are summarized in combined scatter and box plots. Box = 25–75% of percentile, whisker = 1.5 interquartile range, − = median, □ = mean value, X = minimum/maximum value. The Kruskal–Wallis ANOVA with subsequent Dunn’s test was used to test for statistical significance. Asterisk indicates statistical significance (α = 0.01). mV = mVenus; mCh = mCherry; SCN = stem cell niche.