Figure EV2. CK2 inhibitor does not affect starvation‐induced autophagy.

HEK293T cells stably expressing the GFP‐LC3‐RFP autophagic flux reporter were cultured with 10 µM or 20 µM CK2 inhibitor for 3 h followed by starvation (with the same concentration of inhibitor) for 6 h and analyzed by flow cytometry. The GFP:RFP ratio was quantified. Data represent the mean ± SEM of four independent experiments. Differences were statistically analyzed by one‐way ANOVA and Tukey’s multiple comparison test.