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. 2022 May 17;23(6):e53791. doi: 10.15252/embr.202153791

Figure EV5. Expression of IL‐1Rrp2 in tissues and cell lines and the effect of IL‐38 on JNK activation in A431 cell line.

Figure EV5

  • A, B
    Relative expression of IL‐1Rrp2 in human normal tissues (A) or cell lines (B) based on TPM values. Data were obtained from the Human Protein Atlas Dataset available from proteinatlas.org.
  • C
    Cell extracts prepared from A431 cells transfected with a mock mammalian expression vector (left) or an expression vector encoding IL‐38 (right), were blotted with anti‐phospho‐JNK, anti‐JNK, or anti‐β‐actin antibodies.
  • D
    Cell extracts prepared from A431 cells infected with lentiviruses containing Negative Control shRNA (left) or IL‐38 shRNA (right) were probed using anti‐phospho‐JNK, anti‐JNK, or anti‐β‐actin antibodies.
  • E
    The expression of IL‐1Rrp2 in A431 cells transfected with IL‐1Rrp2‐siRNA was detected using western blot.
  • F
    The expression of JNK in A431 cells transfected with JNK‐siRNA was detected using western blot.