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. 2022 May 24;16:866020. doi: 10.3389/fncel.2022.866020

FIGURE 2.

FIGURE 2

miR-582-5p promotes neurosphere formation and proliferation of NS/PCs in vitro. (A) Bright field microscopy of neurosphere assay in mimic/inhibitor group and negative control (NC) group, n = 6, Scale bar = 500 μm. (A’) Corresponding quantifications and statistics of neurosphere number. (B) EdU labeling of mimic/inhibitor group and NC group, n = 6, Scale bar = 100 μm, 50 μm. (B’) Corresponding quantifications and statistics of EdU labeling. (C) RT-qPCR of miR-582-5p and stemness markers Sox2, Nestin, Pax6, CD133, glast, and Vimentin in mimic/inhibitor group and NC group, n = 6. (D) Western blotting of Sox2, Nestin, and Pax6 in mimic/inhibitor group and NC group, n = 6. (D’) Corresponding quantifications and statistics of (D). For statistical analysis, *p < 0.05, **p < 0.01, ***p < 0.001, compared with the control, respectively.