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. 2022 May 23;9:823723. doi: 10.3389/fnut.2022.823723

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Copyright © 2022 Wu, Lin, Pai, Chen, Cheng, Kuo, Li and Ou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Supplemen of sucralose in high-fat diet showed no significant effects on the body weight of mice. Eight-week-old C57BL/6 mice were fed with a chow diet (Chow), high-fat diet (HFD), or HFD supplemented with sucralose (HFSUC) for 12 weeks, and the body weight (A), food intake (B), and water intake (C) were measured using metabolic cages. At the end of the experiments, each group of the mice was sacrificed and the epididymal fat pads (eWAT) were removed and weighed (D). Data are mean ± SEM (n = 7–8 for each group of the mice) ^***p < 0.001 as compared with the Chow group.