FIGURE 2.

Supplement of sucralose exacerbated high fat diet-induced hepatic steatosis in mice. Eight-week-old C57BL/6 mice were fed with a chow diet (Chow), high-fat diet (HFD), or HFD supplemented with sucralose (HFSUC) for 12 weeks. At the end of the experiments, each group of the mice was sacrificed and the liver tissues were removed and weighed (A). Hepatic triglyceride contents for each group of the mice were measured using a commercialized assay kit (B). In addition, the liver sections were stained with hematoxylin and eosin (100X) (C). Serum samples were collected for the determination of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels (D). Data are mean ± SEM (n = 7–8 for each group of the mice) *p < 0.05, ^**p < 0.01, ^***p < 0.001 as compared with the Chow group or indicated groups.