Table 3.
Fermentation experiments performed on Biostat CT plus (3 L) and Biostat 100 (150 L) bioreactors.
| Process | OD max (600 nm) | Viability (CFU/mL) | Dry weight (g/L) | LA (g/L) | Et (g/L) | Yx/s (g/g) | YLA/x (g/g) | YEt/x (g/g) | EPS (glucose equivalents) (mg/mL) |
|---|---|---|---|---|---|---|---|---|---|
| Batch | |||||||||
| 10 g/L | 5.1 ± 0.4 | 3.6 ± 0.6 × 108 | 1.7 ± 0.1 | 6.5 ± 0.7 | 3.0 ± 0.6 | 0.17±0.04 | 3.8 ± 0.1 | 1.7 ± 0.2 | n.d |
| 30 g/L | 6.3 ± 0.3 | 1.0 ± 0.1 × 109 | 2.4 ± 0.3 | 19.5 ± 1.3 | 6.7 ± 1.4 | 0.09±0.01 | 8.3 ± 1.3 | 2.8 ± 0.7 | 0.450±0.040 |
| 50 g/L | 7.7 ± 1.5 | 6.9 ± 1.7 × 108 | 2.7 ± 0.5 | 26.8 ± 1.5 | 9.1 ± 1.1 | 0.07±0.001 | 9.8 ± 1.1 | 3.4 ± 0.2 | 0.630±0.006 |
| Batch_P | 7.3 ± 0.2 | 6.1 ± 0.9 × 108 | 2.7 ± 0.3 | 34.5 ± 0.7 | 9.6 ± 0.8 | 0.05±0.006 | 12.8 ± 1.7 | 3.6 ± 0.7 | 0.400±0.004 |
| Scale up | |||||||||
| Batch 150L | 6.8 | 8.0 × 108 | 2.6 | 21.4 | 8.2 | 0.10 | 8.2 | 3.1 | 0.47 |
EPS, exopolysaccharides; Batch_P, batch with pulse; LA, lactic acid; Et, ethanol.