TABLE 1.

Production of exopolymers with glucose and its analogs by A. pullulans^a

Carbon source	Final pHb	Cell dry wt (mg/ml)	Yield (mg/ml)c	Specific yield (mg/mg [dry wt] of cells)d	Mol% of:
					Glucosee	Mannosef
Glucose (2%)	6.19	9.79	3.21 ± 0.35	0.34	87 ± 3	13 ± 3
3-O-Methylglucose (2%)	7.47	1.74	0.47 ± 0.10	0.36	57 ± 6	43 ± 6
Glucosamine (2%)	5.75	3.94	0.70 ± 0.11	0.21	30 ± 7	70 ± 7
N-Acetylglucosamine (2%)	7.62	3.15	0.51 ± 0.12	0.22	43 ± 4	57 ± 4
2-Deoxyglucose (2%)	6.82	0.43	0.35 ± 0.08	1.09	54 ± 5	46 ± 5
Mannose (2%)	5.40	7.56	2.63 ± 0.07	0.35	89 ± 4	11 ± 4

^aThe data are data for 5-day cultures grown at 30°C, and the values are means based on triplicate experiments. The values for controls included to account for medium or exopolymer carryover were as follows: for cell dry weight, 0.23 ± 0.00 mg/ml; and for yield, 0.17 ± 0.01 mg/ml. The control values should be subtracted from the gross values shown. 

^bThe initial medium pH was adjusted to 6.5 to 6.7 prior to sterilization. 

^cTotal amount of exopolymers, including pullulan. 

^dSpecific yield of exopolymers, including pullulan. 

^eMoles percent of glucose = [glucose/(glucose + mannose)] × 100. 

^fMoles percent of mannose = [mannose/(glucose + mannose)] × 100.