TABLE 2.

Production of exopolymers with mixed carbon sources by A. pullulans^a

Concn (%) of Carbon sources		Final pHb	Cell dry wt (mg/ml)	Yield (mg/ml)c	Specific yield (mg/mg [dry wt] of cells)d	Mol% of
Glucose	Glucosamine					Glucosee	Mannosef
2.0	0.0	5.80	9.61	3.10 ± 0.30	0.31	87 ± 3	13 ± 3
1.5	0.5	4.86	8.07	2.29 ± 0.03	0.28	80 ± 2	20 ± 2
1.0	1.0	5.80	8.33	1.60 ± 0.05	0.19	75 ± 3	25 ± 3
0.5	1.5	5.80	8.30	1.14 ± 0.05	0.14	61 ± 3	39 ± 3
0.0	2.0	5.80	3.57	0.74 ± 0.02	0.21	28 ± 2	72 ± 2

^aThe data are data for 5-day cultures grown at 30°C, and the values are means ± standard deviations based on results of triplicate experiments. 

^bThe initial medium pH was adjusted to 6.5 to 6.7 prior to sterilization. 

^cTotal amount of exopolymers, including pullulan. 

^dSpecific yield of exopolymers, including pullulan. 

^eMoles percent of glucose = [glucose/(glucose + mannose)] × 100. 

^fMoles percent of mannose = [mannose/(glucose + mannose)] × 100.