Table 2.
DNA extraction methods and sequencing of CO1 DNA barcodes of mosquitoes from the Jazan region (processed at NEA—Singapore).
| Identified Mosquito Species | No. of Specimens | Tissue Sampled | PCR | Sequencing | Remarks |
|---|---|---|---|---|---|
| Aedes aegypti | 6 | 2–3 legs | Success | Success | Adult reared from larvae in the lab |
| Aedes vexans | 2 | 2–3 legs | Success | Success | Adult collected from the field |
| Aedes vexans | 3 | Head + thorax | Success | Success | Adult collected from the field |
| Aedes vexans | 1 | Whole mosquito | Success | Success | Adult reared from larvae in the lab |
| Aedes vexans | 1 | Whole mosquito | Success | Fail | Adult reared from larvae in the lab |
| Aedes vexans | 4 | Head + thorax | Faint band | - | Old preserved specimen |
| Aedes vexans | 6 | 2–3 legs | No bands | - | Old preserved specimen |
| Aedes vexans | 2 | Head + thorax | No bands | - | Old preserved specimen |
| Aedes vexans | 2 | Whole mosquito | No bands | - | Old preserved specimen |
| Aedes vittatus | 1 | 2–3 legs | Success | Success | Adult reared from larvae in the lab |
| Aedes vittatus | 1 | Whole mosquito | Success | Success | Adult reared from larvae in the lab |
| Aedes vittatus | 1 | 2–3 legs | No bands | - | Old preserved specimen |
| Anopheles arabiensis | 2 | Whole mosquito | Success | Success | Adult reared from larvae in the lab |
| Anopheles arabiensis | 2 | Whole mosquito | Success | Fail | Adult reared from larvae in the lab |
| Anopheles arabiensis | 4 | 2–3 legs | No bands | - | Old preserved specimen |
| Anopheles dthali | 2 | 2–3 legs | No bands | - | Old preserved specimen |
| Anopheles fluviatilis | 1 | Head + thorax | No bands | - | Old preserved specimen |
| Anopheles multicolor | 1 | 2–3 legs | No bands | - | Old preserved specimen |
| Anopheles multicolor | 1 | Whole mosquito | No bands | - | Old preserved specimen |
| Anopheles pretoriensis | 2 | Whole mosquito | No bands | - | Old preserved specimen |
| Anopheles sergenti | 1 | Thorax | No bands | - | Old preserved specimen |
| Anopheles stephensi | 1 | 2–3 legs | No bands | - | Old preserved specimen |
| Anopheles turkhudi | 1 | Whole mosquito | No bands | - | Old preserved specimen |
| Culex pipiens | 2 | 2–3 legs | Success | Success | Adult reared from larvae in the lab |
| Culex pipiens | 3 | Whole mosquito | Success | Success | Adult reared from larvae in the lab |
| Culex quinquefasciatus | 2 | 2–3 legs | Success | Success | Adult reared from larvae in the lab |
| Culex quinquefasciatus | 2 | 2–3 legs | Double bands | - | Adult reared from larvae in the lab |
| Culex quinquefasciatus | 1 | Whole mosquito | No bands | - | Old preserved specimen |
| Toxorhynchites spp. | 2 | 2–3 legs | No bands | - | Old preserved specimen |
| Total | 60 | - | - | 23 |