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Effect of addition of peptone (0.5%, wt/vol) at high osmolarity on the growth of an E. coli strain deficient in the three proline permeases (EF063). Cells were grown to exponential phase in McIlvaine’s medium exactly as described in Materials and Methods. (a) EF063, 0.5 M NaCl (○); 0.5 M NaCl plus 0.5% (wt/vol) peptone (▴); and 0.5 M NaCl plus 1 mM proline (●). (b) MK11 (isogenic strain with ProP activity). Symbols are the same as in panel a.
