Table 1.
Thermodynamic binding parameters determined by ITC
| Construct* | KD (nM) | Stoichiometry | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) |
|---|---|---|---|---|---|
| 321 to 397 (3) (WT fragment) | 15 ± 6 | 0.79 ± 0.02 | −25.2 ± 1.0 | 14.6 ± 1.2 | −10.6 ± 0.3 |
| Δloop (9) (deletion/substitution) | 7.3 ± 4.1 | 0.92 ± 0.22 | −19.1 ± 4.3 | 8.1 ± 4.1 | −11.0 ± 0.4 |
| LI > EE (4) (substitutions) | 65 ± 10 | 0.76 ± 0.04 | −22.8 ± 1.0 | 13.1 ± 1.1 | −9.6 ± 0.1 |
| QLE > GGG (5) (substitutions) | 47 ± 17 | 0.75 ± 0.10 | −20.7 ± 3.1 | 10.8 ± 2.9 | −10.0 ± 0.2 |
| I327R (3) (substitution) | 449 ± 60 | 1.09 ± 0.14 | −17.5 ± 1.4 | 8.9 ± 1.4 | −8.5 ± 0.1 |
| I327W (4) (substitution) | 29 ± 4 | 0.82 ± 0.01 | −21.7 ± 0.2 | 11.6 ± 0.3 | −10.1 ± 0.1 |
| EE > RR (4) (substitutions) | 34 ± 10 | 0.99 ± 0.22 | −19.0 ± 3.4 | 9.0 ± 3.3 | −10.0 ± 0.2 |
| 3R (4) (substitutions) | 275 ± 59 | 1.21 ± 0.04 | −14.2 ± 0.6 | 5.4 ± 0.6 | −8.8 ± 0.2 |
Data are reported as mean ± SD.
*The number of measurements is indicated in parentheses.