Graphs showing morphometric differences between RPE subpopulations in AMD and non-AMD donor eyes. (A, C, E, and G) Quantification of each RPE subpopulation for cell area (square micrometers) (A); AR (C); hexagonality (E); and number of neighbors (G) presented as boxplots (box limits represent the first and third quartile, the central line shows the median and the whiskers indicate the 5th and 95th percentile. **P < 0.01, *P < 0.05). (B, D, F, and H) Corresponding statistical analysis table for cell area (B), AR (D), hexagonality (F), and number of neighbors (H) presented for pairwise comparisons with Tukey test. (A linear mixed-effects model and a Tukey test for multiple comparisons were performed, n = 5; **P < 0.01, *P < 0.05; SI Appendix, Tables S16–S18). (I, K, M, and O) Quantification of each RPE subpopulation for cell area (square micrometers) (I), AR (K), hexagonality (M), and number of neighbors (O) for AMD (red) and non-AMD donors (blue) are presented side by side to show the differences (the error bars indicate the 5th and 95th percentile). (J, L, N, and P) Corresponding tables of statistical analysis for cell area (J), AR (L), hexagonality (N), and number of neighbors (P) where AMD and non-AMD measurements for each RPE subpopulation were compared with t test; *P < 0.05. HSD, honest significant difference.