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. 2022 May 6;119(19):e2117553119. doi: 10.1073/pnas.2117553119

Fig. 5.

Fig. 5.

Far-peripheral RPE subpopulation P5 contains sub-RPE deposits reminiscent of macular RPE P1. (A and B) Low- and high-magnification (Inset) images of P4 RPE subpopulation stained with phalloidin (white) dye (A) and RPE cell areas color coded (B) reveal lesions in non-AMD donor RPE flatmounts. (C) Three-dimensional imaging of a piece of retina and RPE over the area of RPE lesion stained with phalloidin (white) and PNA (magenta) dyes reveals intact RPE and photoreceptors in the area of lesions. Asterisks mark sub-RPE deposits in the lesion area (n = 3 donors). (D and E) The quantity of lesioned (lifted) RPE for each population was calculated as a percentage of the area of not-segmented cells for both non-AMD (D) and AMD donor (E) eyes. Far-peripheral subpopulation P5 contains more lesions than midperipheral population P3 in non-AMD eyes (n = 17). The same tendency is observed in AMD eyes, although the variability between each donor is greater (n = 5) (box limits represent the first and third quartile, the central line shows the median and the whiskers indicate the 5th and 95th percentile. One-way ANOVA and Tukey test for multiple comparisons were performed; *P < 0.05). Note that for AMD eyes our quantification does not distinguish between geographic atrophies and lesions due to sub-RPE deposits. ONL, outer nuclear layer.