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. 2022 May 8;23(6):e54157. doi: 10.15252/embr.202154157

Figure EV4. Characterization of the Ntras CA‐repeat in vivo .

Figure EV4

  1. Illustration of the murine Ntras locus. Displayed is Ntras (GRCm38/mm10; 1700034H15) and RBPmap‐predicted hnRNPL binding motifs, described elsewhere (Smith et al, 2013).
  2. Genotyping results confirming the genomic deletion of the hnRNPL binding motif in Ntras ∆CA/∆CA mice.
  3. RT–qPCR‐based analysis of Ntras expression in Ntras CA/CA and Ntras ∆CA/∆CA mice (n = 5–11 mice per group).
  4. Western blot‐based analysis of the NTRAS–hnRNPL interaction following antisense affinity selection of NTRAS in nuclear fractions from NTRAS CA controls and NTRAS ∆CA HeLa cells (n = 3 independent biological replicates). Representative western blot on the right.

Data information: In (C, D), data are represented as mean ± SEM. n.s.: non‐significant, *P < 0.05. (C, D) two‐tailed unpaired t‐test.

Source data are available online for this figure.