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. 2022 Apr 19;23(6):e54275. doi: 10.15252/embr.202154275

Figure EV3. iHCC samples express HCC markers.

Figure EV3

  1. Representative IHC images of four iHCC samples (iHCC1‐1, iHCC2‐1, iHCC3‐1, and iHCC4‐1) from NSIF mice transplanted with MTK‐transduced PHHs from four different PHH donors (PHH1~4) and an HCC tissue from a patient (pHCC2). Anti‐HLA‐ABC, anti‐ALB, anti‐AFP, anti‐GPC3 and anti‐KRT19 antibodies were used. Scale bars, 20 μm.
  2. DNA sequencing confirmed mutations of TP53 in the genomic DNA of sgTP53 transduced PHH cells.
  3. Expression of p53 was detected in the PHH cells transduced with sgMock or sgTP53 by Western blotting.
  4. Representative images of in situ liver carcinomas derived from PHHs transduced with a combination of MTK with or without deletion of TP53 in NSIF mice. 3 out of 4 mice harbored tumor in both MTK+ sgMock and MTK+ sgTP53 groups after 11 weeks (n = 4 per group). N‐numbers refer to biological replicates.
  5. Representative IHC images Ki67 staining of liver sections from NSIF mice transplanted with PHHs that were transduced with the combinations of MYC/TP53R249S/KRASG12D (MTK) or MYC/TP53R249S (MT) lentiviruses. Scale bars, 20 μm.

Source data are available online for this figure.